REAL-TIME RT-PCR HIGH-RESOLUTION MELTING CURVE ANALYSIS TO DETECT AND DIFFERENTIATE BRAZILIAN VARIANTS OF GRAPEVINE VIRUSES

Real-time RT-PCR high-resolution melting curve analysis to detect and differentiate Brazilian variants of grapevine viruses

Detecting and identifying china glaze holee shift viral infections in perennial plants, such as grapevines, can be challenging.Therefore, the aim of this study was to perform a real-time RT-PCR (RT-qPCR) high-resolution melting (HRM) curve analysis to detect and differentiate Brazilian variants of grapevine leafroll-associated virus 3 (GLRaV-3) and

read more

Educational Metaphor of Journey

Metaphor has many capacities in conjunction with different meanings and can bring a more comprehensive concept of common terms to mind.Hence, in the present study, education is considered as an internal journey which is titled "Educational Metaphor of Journey".Accordingly, the present study aims at explaining the educational metaphor of the journey

read more

Corpus callosotomy in pediatric patients with non-lesional epileptic encephalopathy with electrical status epilepticus during sleep

Epileptic encephalopathy with electrical status epilepticus during sleep (ESES) is often refractory to medical treatment and leads to poor cognitive outcomes.Corpus callosotomy may be an effective treatment option for drug-resistant ESES with no focal etiology.We retrospectively identified three patients who underwent corpus callosotomy for drug-re

read more

Does DNA methylation mediate the association of age at puberty with forced vital capacity or forced expiratory volume in 1 s?

Background Age of pubertal onset is associated with lung function in adulthood.However, the underlying role of epigenetics as a mediator of this association remains unknown.Methods DNA methylation (DNAm) in peripheral blood was measured at age 18 years in the Isle of Wight birth cohort (IOWBC) along with data on age of pubertal events, forced vital

read more